Enzyme linked immunosorbent assay (ELISA) is a biochemical technique used mainly in immunology to detect the presence of an antibody or an antigen in a sample. In the ELISA technique, an antigen or antibody is bound to a solid surface and then treated with an enzyme-labeled antibody or antigen, respectively. Following this, a substrate for the enzyme is added and the sample is examined for a color change, which indicates the presence of an antigen-antibody complex and therefore a positive result.
History and Background
Enzyme Linked Immunosorbent Assay was developed in the 1970s as an improvement to the radioimmunoassay (RIA) technique. Unlike RIA, which uses radioisotopes as tracers, ELISA uses enzymatic reactions, making it safer, easier to handle, and more stable than RIA. The first description of ELISA was published in 1971 by Peter Perlmann and Eva Engvall at the University of Uppsala, Sweden. Since then, ELISA has become one of the most widely used and powerful techniques for detecting antibodies, antigens, and other biomolecules.
Get More Insights On-Enzyme Linked Immunosorbent Assay
Resources:
Understanding the Key Applications of Enzyme Linked Immunosorbent Assay in Diagnostics
How Enzyme Linked Immunosorbent Assay Revolutionizes Immunoassay Techniques
